Sperm penetration assay

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The sperm penetration assay, or zona-free hamster oocyte penetration assay is utilized to measure the ability of sperm to undergo capacitation, fuse with the egg membrane and decondense the sperm head within the cytoplasm of the oocyte resulting in the formation of the male pronucleus. The test is scored by calculation the percentage of ova that are penetrated or the average number of sperm penetrations per ovum. It has been used to identify those couples who will have a high likelihood of success with in vitro fertilization. Infertility is a difficult and stressful condition for both the patients and the treating physicians as well.
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Sperm Penetration Assay

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Sperm penetration assay | definition of sperm penetration assay by Medical dictionary

Top of the page. Sperm penetration tests check to see if a man's sperm can move through cervical mucus and the fallopian tubes to join with fertilize an egg. This test is usually done when a couple is having trouble getting pregnant infertility. Talk to your doctor about any concerns you have about the need for the test, its risks, how it will be done, or what the results will mean.
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The Sperm Penetration Assay for the Assessment of Fertilization Capacity

A sperm penetration assay SPA is a lab test used to measure sperm ability to undergo capacitation— a set of physiological changes that sperm must undergo to be able to penetrate the egg and fertilize it. The test calculates the percentage of pretreated hamster eggs that are penetrated by human sperm. SPA is not frequently performed and is unlikely to significantly alter treatment recommendations. Sperm penetration assay is also known as a hamster test, hamster penetration test, and zona-free hamster oocyte penetration assay. In order to predict the capacity of sperm to fertilize an egg, a sample of sperm is mixed with prepared hamster eggs in a laboratory.
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